首页 > 物质学院 > 师资队伍 > 季泉江个人简介
季泉江    助理教授、研究员
所在学院 物质科学与技术学院
研究方向 化学生物学和合成生物学
联系方式 quanjiangji@@shanghaitech.edu.cn
备注 青年千人
 
  个人简介  
2005年-2009年就读于南京大学化学系获得理学学士学位;
2009年-2014年就读于芝加哥大学化学系,获得化学博士学位(导师为何川教授);
2014年-2016年在加州大学伯克利分校化学系从事博士后研究(合作教授为Michelle Chang 教授);
2016年2月加入上海科技大学物质科学和技术学院,任助理教授。
曾经荣获2013年国家优秀自费留学生奖,Camille and Henry Dreyfus Postdoc Fellow, Everett E Gilbert Memorial Prize等奖项。2016年入选第十二批“青年千人”。
  主要研究内容  
研究组以当代社会所面临的能源,环境和健康等方面的重大问题为导向,利用合成生物学和化学生物学等手段,致力于下一代生物能源生产工程菌株,环境污染物富集或降解工程菌株的定向进化和相关进化技术的开发,以及重要人类致病菌致病机制探索与小分子药物开发等方面的研究。

(1)致病菌基因编辑新技术
CRISPR/Cas9是最新发现的一种高效快速的基因编辑技术。课题组将该项技术应用到人类的致病细菌中,构建能够在各种致病菌中进行高效基因组编辑的工具,为研究致病菌感染和抗药机制,药物靶标筛选等方面提供技术支持。


(2)致病菌感染机制探究和药物开发
研究致病菌中与感染,耐药等相关的蛋白与信号通道,通过解析相应关键蛋白及其与小分子复合物的晶体结构,从分子层面研究细菌的致病机理和潜在的药物靶点,为设计抗菌药物提供分子层面的支持。同时利用化学生物学、分子生物学和遗传学等手段从生化和表型等方面研究致病菌的感染机制。基于结构信息,理性设计和筛选新型小分子药物。


(3)基因簇抓取
基因簇(gene cluster)指细菌中一组紧密连锁的且功能上密切相关的结构基因。这些基因各自编码的酶通常能组成多酶复合物,催化生命代谢中的特定反应。课题组致力于基于CRISPR基因组编辑技术的新型基因簇抓取和功能研究的技术开发,为研究基因簇上各基因功能等方面提供有效工具。


(4)基因组进化
高效工业菌株的获得往往需要对原始菌株进行一系列复杂繁琐的进化。课题组致力于高效基因组进化和蛋白质进化技术开发,并尝试这些技术在二代生物能源工程菌株进化中的应用。
  代表性论文  
20. Chen, W, Zhang, Y, Yeo, WS, Bae, T, Ji, Q. Rapid and efficient genome editing in Staphylococcus aureus by using an engineered CRISPR/Cas9 system. J Am Chem Soc DOI: 10.1021/jacs.6b13317 (2017).


19. Lin R, Elf S, Shan C, Kang HB, Ji Q, Zhou L, Hitosugi T, Zhang L, Zhang S, Seo JH, Xie J, Tucker M, Gu TL, Sudderth J, Jiang L, Mitsche M, DeBerardinis RJ, Wu S, Li Y, Mao H, Chen PR, Wang D, Chen GZ, Hurwitz SJ, Lonial S, Arellano ML, Khoury HJ, Khuri FR, Lee BH, Lei Q, Brat DJ, Ye K, Boggon TJ, He C, Kang S, Fan J, Chen J. 6-Phosphogluconate dehydrogenase links oxidative PPP, lipogenesis and tumour growth by inhibiting LKB1-AMPK signaling. Nat Cell Biol. 17, 1484-96 (2015).

18. Kang HB, Fan J, Lin R, Elf S, Ji Q, Zhao L, Jin L, Seo JH, Shan C, Arbiser JL, Cohen C, Brat D, Miziorko HM, Kim E, Abdel-Wahab O, Merghoub T, Fröhling S, Scholl C, Tamayo P, Barbie DA, Zhou L, Pollack BP, Fisher K, Kudchadkar RR, Lawson DH, Sica G, Rossi M, Lonial S, Khoury HJ, Khuri FR, Lee BH, Boggon TJ, He C, Kang S, Chen J. Metabolic Rewiring by Oncogenic BRAF V600E Links Ketogenesis Pathway to BRAF-MEK1 Signaling. Mol Cell. 59, 345-58 (2015).

17. Deng X, Chen K, Luo GZ, Weng X, Ji Q, Zhou T, He C. Widespread occurrence of N6-methyladenosine in bacterial mRNA. Nucleic Acids Res. 43, 6557-67 (2015).

16. Fu Y, Luo GZ, Chen K, Deng X, Yu M, Han D, Hao Z, Liu J, Lu X, Doré LC, Weng X, Ji Q, Mets L, He C. N6-methyldeoxyadenosine marks active transcription start sites in Chlamydomonas. Cell. 161, 879-92 (2015).

15. Shan C, Elf S, Ji Q, Kang HB, Zhou L, Hitosugi T, Jin L, Lin R, Zhang L, Seo JH, Xie J, Tucker M, Gu TL, Sudderth J, Jiang L, DeBerardinis RJ, Wu S, Li Y, Mao H, Chen PR, Wang D, Chen GZ, Lonial S, Arellano ML, Khoury HJ, Khuri FR, Lee BH, Brat DJ, Ye K, Boggon TJ, He C, Kang S, Fan J, Chen J. Lysine acetylation activates 6-phosphogluconate dehydrogenase to promote tumor growth. Mol Cell. 55, 552-65 (2014).

14. Deng X, Liang H, Ulanovskaya OA, Ji Q, Zhou T, Sun F, Lu Z, Hutchison AL, Lan L, Wu M, Cravatt BF, He C. Steady-state hydrogen peroxide induces glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa. J Bacteriol. 196, 2499-513 (2014).

13. Chen W, Zhang L, Zheng G, Fu Y, Ji Q, Liu F, Chen H, He C. Crystal structure of the RNA demethylase ALKBH5 from zebrafish. FEBS Lett. 588, 892-8 (2014).

12. Ji Q, Zhao BS, He C. A highly sensitive and genetically encoded fluorescent reporter for ratiometric monitoring of quinones in living cells. Chem Commun. 49, 8027-9 (2013).

11. Deng X, Weerapana E, Ulanovskaya O, Sun F, Liang H, Ji Q, Ye Y, Fu Y, Zhou L, Li J, Zhang H, Wang C, Alvarez S, Hicks LM, Lan L, Wu M, Cravatt BF, He C. Proteome-wide quantification and characterization of oxidation-sensitive cysteines in pathogenic bacteria. Cell Host Microbe. 13, 358-70 (2013).

10. Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, Cho H, Brugarolas P, Gao YN, Peterson SN, Lan L, Bae T, He C. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proc Natl Acad Sci U S A. 110, 5010-5 (2013).

9. Liang H, Deng X, Bosscher M, Ji Q, Jensen MP, He C. Engineering bacterial two-component system PmrA/PmrB to sense lanthanide ions. J Am Chem Soc. 135, 2037-9 (2013).

8. Sun F, Ding Y, Ji Q, Liang Z, Deng X, Wong CC, Yi C, Zhang L, Xie S, Alvarez S, Hicks LM, Luo C, Jiang H, Lan L, He C. Protein cysteine phosphorylation of SarA/MgrA family transcriptional regulators mediates bacterial virulence and antibiotic resistance. Proc Natl Acad Sci U S A. 109, 15461-6 (2012).

7. Jeong DW, Cho H, Jones MB, Shatzkes K, Sun F, Ji Q, Liu Q, Peterson SN, He C, Bae T. The auxiliary protein complex SaePQ activates the phosphatase activity of sensor kinase SaeS in the SaeRS two-component system of Staphylococcus aureus. Mol Microbiol. 86, 331-48 (2012).

6. Sun F, Liang H, Kong X, Xie S, Cho H, Deng X, Ji Q, Zhang H, Alvarez S, Hicks LM, Bae T, Luo C, Jiang H, He C. Quorum-sensing agr mediates bacterial oxidation response via an intramolecular disulfide redox switch in the response regulator AgrA. Proc Natl Acad Sci U S A. 109, 9095-100 (2012).

5. Ji Q, Zhang L, Sun F, Deng X, Liang H, Bae T, He C. Staphylococcus aureus CymR is a new thiol-based oxidation-sensing regulator of stress resistance and oxidative response. J Biol Chem. 287, 21102-9 (2012).

4. Liang H, Deng X, Ji Q, Sun F, Shen T, He C. The Pseudomonas aeruginosa global regulator VqsR directly inhibits QscR to control quorum-sensing and virulence gene expression. J Bacteriol. 194, 3098-108 (2012).

3. Deng X, Sun F, Ji Q, Liang H, Missiakas D, Lan L, He C. Expression of multidrug resistance efflux pump gene norA is iron responsive in Staphylococcus aureus. J Bacteriol. 194, 1753-62 (2012).

2. Sun F, Ji Q, Jones MB, Deng X, Liang H, Frank B, Telser J, Peterson SN, Bae T, He C. AirSR, a [2Fe-2S] cluster-containing two-component system, mediates global oxygen sensing and redox signaling in Staphylococcus aureus. J Am Chem Soc. 134, 305-14 (2012).

1. Ding L, Ji Q, Qian R, Cheng W, Ju H. Lectin-based nanoprobes functionalized with enzyme for highly sensitive electrochemical monitoring of dynamic carbohydrate expression on living cells. Anal Chem. 82, 1292-8 (2010).

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